The total photosynthetic rate in leaf mustard lines, which was calculated as the sum of the photosynthetic rate and the respiration rate, was not significantly different from their photosynthetic rate. Plant height, standing of rosetteness, showed a similar change to its specific leaf area (SLA). With increasing the plant height, leaf density increased and leaf color was lighter. It was found that shoot dry weight of leaf mustard was more affected by respiration. Also, it was hypothesized that respiration occurred not only in the leaf but also the stem. It was found that mustard lines whose leaf density was low showed a higher shoot growth. From this result, it was concluded that selection of a leaf mustard line with a larger SLA and lower leaf thickness could be effective in increasing photosynthetic rate.