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Determination of Capsaicinoids in Foods Using Ultra High Performance Liquid Chromatography
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  • Determination of Capsaicinoids in Foods Using Ultra High Performance Liquid Chromatography
  • Determination of Capsaicinoids in Foods Using Ultra High Performance Liquid Chromatography
저자명
Ha. Jae-Ho,Seo. Hye-Young,Shim. You-Shin,Seo. Dong-Won,Seog. Ho-Moon,Ito. Masahito,Nakagawa. Hiroaki
간행물명
Food science and biotechnology
권/호정보
2010년|19권 4호|pp.1005-1009 (5 pages)
발행정보
한국식품과학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

A sensitive, precise, and specific ultra high performance liquid chromatographic (u-HPLC) method was developed for the analysis of capsaicin in foods. The method validation parameters yielded good results, including linearity, precision, accuracy, and recovery. The u-HPLC separation was performed on a reversed column $C_{18}$ (particle size 2 ${mu}m$, i.d. 2 mm, length 50 mm, followed by fluorescence detection-excitation 280 nm, emission 325 nm). The recovery of capsaicin in gochujang was more than 91%, and the detection limit and lower determination limit of u-HPLC analysis were 0.054 and 0.163 ${mu}g/mL$ for capsaicin and 0.053 and 0.160 ${mu}g/mL$ for dihydrocapsaicin, respectively. The calibration graph for capsaicin and dihydrocapsaicin was linear from 0.2 to 10.0 ${mu}g/mL$ for u-HPLC analysis. The inter-day and intra-day precisions (relative standard deviations) were <5.21% for capsaicin and <9.79% for dihydrocapsaicin while the average recoveries obtained were quantitative 91.1-94.8% for capsaicin, 91.4-97.0% for dihydrocapsacin, indicating good accuracy of the u-HPLC method.