Textile processes generally produce a large amount of wastewater and cause a negative environmental impact. The use of proteases in wool finishing could be an appropriate alternative to classical finishing methods. However, the enzymatic treatment could cause excessive fiber damage. The application of biopolymer chitosan on wool fabrics prior to proteases treatment in attempt to overcome the damage promoted by the enzymes has been studied. The treatments based on chitosan application followed by enzymatic treatment reduce felting shrinkage, enhance whiteness degree, and improve dyeability of wool. Moreover, it plays an important role in minimizing the wool fiber damage.