In the field of yarn spinning engineering, the importance of the processing parameters taken depends directly on the quality characteristics of the yarn. This study aimed to find the optimal processing parameters for an open-end rotor spinning frame at work to identify its multiple quality characteristics for yarn. In this study, Bamboo charcoal and cotton 70 %/polyester 30 % (CVC) blended fibers were adopted as the materials, and the open-end rotor spinning frame was used to spin the yarn. In order to identify optimal conditions of an open-end rotor spinning frame, the Taguchi experimental method was applied to design open-end rotor spinning experiments, and the $L_9$ orthogonal array was chosen in accordance with nine sets of experiments and contained four control factors and three levels. Furthermore, a response surface methodology (RSM) was used to obtain the models of significant processing parameters for the strength, unevenness, I.P.I, and hairiness. Based on experiments designed to obtain an open-end rotor spun yarn Ne 30, the strength, unevenness, imperfection indicator/km (I.P.I) and hairiness were then chosen as the quality characteristics. In addition, grey relational analysis integrated the optimal processing parameter of multiple quality characteristics, and a confirmation experiment was performed. In conclusion, the optimal processing parameters under steady spinning conditions were a rotor speed of 88000 rpm, a feed speed of 0.392 m/min, and a winding speed of 39.466 m/min.