In this study, the structural analysis was performed to optimize the membrane humidifier with hollow fiber membrane for polymer electrolyte membrane fuel cell system. The main design factors were considered by evaluating the humidifying performance according to various structural parameters such as packing density and length. The effects of operation conditions of membrane humidifier were also elucidated experimentally. Results imply that there are optimum points for the packing density and length of humidifier. It was also found that among operation conditions, relative humidity of wet exhaust gas and temperature of dry inlet gas have major effects on the humidifying performance.