The extraction characteristics and physiological properties of Viscum album var. coloratum(mistletoe) were monitored by response surface methodology (RSM). The maximum electron donating ability 88.05% was obtained at 42.10 W of microwave power, 36.99% of ethanol concentration, and 7.17 min of extraction time. The maximum inhibition effect on tyrosinase was 62.88% at microwave power, and its condition was 84.02 W, 92.46%, and 7.07 min of ethanol concentration, and extraction time, respectively. Superoxide dismutase (SOD) showed a maximum pseudo-activity of 53.55% at microwave power, 142.69 W, ethanol concentration, 32.69%, and extraction time, 3.58 min. The maximum total polyphenol content was 72.82% and it was under 122.57 W, 16.81%, and 2.94 min of microwave power, ethanol concentration, and extraction time, respectively. Based on superimposition of four-dimensional RSM regarding electron-donating ability, inhibition effect on tyrosinase, pseudo-activity of SOD, and total polyphenol content, the optimum ranges of extraction conditions are as follows; microwave power, 0-135 W, ethanol concentration, 64-70%, and extraction time, 4.15 min.