An acellular human dermal matrix (ADM) was applied to wounds from dogs with significant dead space and delayed healing. This treatment is typically effective for the treatment of wounds with subcutaneous dead space and injuries between muscular planes. The size of the dead space defect and the amount of wound discharge decreased rapidly with ADM treatment in the present study. The average time to disappearance of the dead space defect was 10 days. In addition, complications including severe inflammation were not seen in this case report.