This study is currently giving priority to developing the ballast water treatment system using ozone(Ozone BWTS). The Ozone BWTS, were equipped on the real merchant vessel named as M. V. Hyundai Hong Kong to carried out shipboard tests of ballast water treatment system in accordance with orders of IMO G8 Guidelines. The study results show that the Ozone BWTS is capable of meeting the Ballast Water Performance Standard under Regulation D-.2 of the International Convention for the Control and Management of Ships’ Ballast Water and Sediments, and also the more stringent standards being proposed under US legislation.