Purpose: The purpose of the study was to identify the effect of humorous video on the depression and stress responses in patients undergoing hemodialysis. Methods: The quasi-experimental study was designed with a nonequivalent control group pre-post test. Research participants were assigned to a treatment group (n=23) and a control group (n=21). Treatment was watching humorous video tapes 30 min each time, three times a week. The depression level and state anxiety level were assessed using a questionnaire. Cortisol was measured by a blood test. The data were analyzed using $x^2$-test, and t-test. Results: Depression and anxiety were decreased significantly in the treatment group. Conclusions: Humorous video tape could be a usefull tool at home and clinical setting as a nursing intervention to decrease depression and anxiety among the patients on hemodialysis.