A method for the identification of structural characteristic parameters of a steel bar in the matrices form such as stiffness matrices and mass matrices from frequency response function(FRF) by genetic algorithm is proposed. As the method is based on the finite element method(FEM), the obtained matrices have perfect physical meanings if the FRFs got from the analysis and the FRFs from the experiments were well coincident each other. The identified characteristic matrices from the FRFs with maximun 40 % of random errors by the genetic algorithm are coincident with the characteristic matrices from exact FEM FRFs well each other. The fitted element diameters by using only 2 points experimental FRFs are similar to the actual diameters of the bar. The fitted FRFs are good accordance with the experimental FRFs on the graphs. FRFs of the rest 9 points not used for calculating could be fitted even well.