In this study, it is conducted that the performance verification of the ambient load carrying capacity algorithm using long-term measurement systems of bridges. For this purpose, a steel-box type model bridge is fabricated and the public load carrying capacity of a steel-box model bridge is estimated by conducting the numerical analysis and load test. In addition, we compare the public load carrying capacity with the estimated result of a steel-box model bridge using the ambient load carrying capacity algorithm. By the assessment result, it is shown that the estimated ambient load carrying capacity is the difference of approximately 6.0 percentages as compared with the public load carrying capacity.