This study aimed to investigate the effect of differing heel height on static balance and muscle activation of ankle joint during standing. Twenty-one young females volunteered to participate in this study. To measure balance function and EMG activity of tibialis anterior and gastrocnemius muscles, the subjects were asked to perform 1-min standing with eyes open and closed state under 3 different heel heights: barefoot, 3cm, and 7cm each. During the standing, postural sway distance and area, and EMG activity of tibialis anterior and gastrocnemius muscles were significantly augmented with increasing heel height (p<0.05). For comparison between eyes open and closed in terms of postural sway area and EMG activity of tibialis anterior muscle, barefoot and 7cm height conditions respectively showed significant differences as well. The findings indicate that high-heeled shoes may have disadvantages in maintaining balance function because of extra-muscular effort of ankle joint. This study provides useful information that will inform future studies on how heel height affects muscle activity around the ankle joint in aspects of static and dynamic balance.