A study of Han River fish assemblage dynamics for 4 years was conducted. From April 2005 to August, 2008, fishes inhabiting two sites of Han River were sampled for identification. For further analysis, 40 individuals of the dominant species were sampled monthly from March 2006 to November 2008. The fish assemblage at site 1 was dominated by Zacco platypus (32.69%), while the subdominant species were Acheilognathus yamatsutae (14.4%), Acanthorhodeus gracilis (9.43%), Squalidus japonicus coreanus (6.84%), and Tridentiger brevispinis (5.18%). The most abundant species at site 2 was Korean Chub (Zacco koreanus) with relative abundance of 62.45% and followed by Pungtungia herzi (10.29%), Coreoperca herzi (8.67%), and Coreoleuciscus splendidus (6.82%) as the subdominant species. At both sites, the endemics populations show an increasing pattern during the whole survey period, while the natives were declining in the last two years.