This study was investigated the effect on operating characteristics of apparatus according to frosting and defrosting to develop of new defrosting equipment. The results showed as following. Frost was almost removed using the defrosting equipment with roll brush type that defrosting is possible under operating condition. Also, the temperature of compressor inlet, evaporator inlet and outlet showed higher value because of heat transfer resistance of cooling pipe frost comparing with defrosting condition. And the compressor work showed 10% lower and COP was presented 24% higher values than defrosting condition. Therefore, defrosting for cooling coil of refrigeration and low temperature storage was effected on operation and performance characteristics of equipment. This highly effects on real refrigeration apparatus which is operated in year-around.