Ammonia ($NH_3$) is an important pollutant that plays a key role in several air pollution problems. It can create odors and have negative impacts on animal and human health. The largest source contributing to Ammonia emission is the agricultural production, in particular animal operation, in Korea. The present study evaluated flux profiles of Ammonia emitted from the cattle housing using a dynamic flux chamber. We have developed the emission factor of Ammonia from the cow housing. Analysis of Ammonia flux variation was made with respect to manure surface temperature, pH, and ammonium concentration. Ammonia has been measured from calf and cattle housing between October and December in 2007. In the fall, average Ammonia flux from calf and cattle housing was estimated 1.342(${pm}0.728$) and 1.323(${pm}0.655$)mg/$m^2$/min, respectively. In the winter, average Ammonia flux was estimated 0.889(${pm}0.362$)mg/$m^2$/min from the calf housing and 0.925(${pm}0.511$)mg/$m^2$/min from the cattle housing. The correlation coefficient between Ammonia flux and ammonium concentration showed stronger relationship than the relationship between manure pH and temperature. In the fall, Ammonia emission factor from calf and cattle housing was estimated 4.46(${pm}2.39$) and 6.03(${pm}3.27$)kg-$NH_3$/animal/yr, respectively. In the winter, average Ammonia flux was estimated 2.88(${pm}1.53$) from the calf housing and 4.24({$pm}1.63$)kg-$NH_3$/animal/yr from the cattle housing.