This study examines if the uniform-related perceptions influence the formation of professional identity. A self-administered survey was conducted on 346 female nursing officers at military hospitals. The independent variables were the basic characteristics, perception of clothing benefit, perception of nursing uniform, and perception of the officer uniform. For the data analysis, we used paired t-test and hierarchical regression analysis. Using a paired t-test, we found the difference in function and satisfaction between nursing uniforms and officer uniforms. The result of hierarchical regression analysis shows that uniform-related perceptions were statistically significant in predicting professional identity. The nursing uniform explained the major portion of variation of professional identity; subsequently, the improvement of uniforms can enhance professional identity.