This study was conducted to determine the susceptibility of 15 clones of native and foreign poplar species and their hybrids to Melampsora leaf rust and to select resistant clones at a nursery stool bed. Rust severities of individual trees were determined by estimating the percentage of infected leaves on the trees in addition to comparing the infected leaves with the infection diagrams. Three hybrids out of 15 clones were selected as resistant clones to the Melampsora leaf rust in Korea. Bong-wha 1 and Hyunsasi 3 belong to section Leuce, and Dorskamp belongs to section Aigeiros. Based on our results, we recommend Dorskamp as the best resistant clone to poplar leaf rust.