A boundary-layer pump with a single disk has been experimented to obtain its characteristic curve by changing the impeller of a centrifugal pump to a single disk. The primary objective to use of these types of pumps is to avoid hurting water life during transportation unnecessarily. The change of impeller should degrade the performance of pump, so we used the method to increase the roughness on the disk with sandpaper and mesh. The enhancement of shear force from the rotation of disk to the internal flow brought an augmentation of momentum transport, and the characteristics were far improved from the original single-disk pump without decreasing the survival rate of water life in the case of Pseudobagrus fulvidraco (bullhead fish). However, in the case of Artemia cyst (zooplankton), the survival rate was very degraded due to the micro scale smaller than turbulent eddy size. The result of this study could be used for the design of transportation and bio-filtering of water lying on a specific bandwidth of its scale of size.