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Purification of Capsular Polysaccharide Produced by Streptococcus pneumoniae Serotype 19A
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  • Purification of Capsular Polysaccharide Produced by Streptococcus pneumoniae Serotype 19A
  • Purification of Capsular Polysaccharide Produced by Streptococcus pneumoniae Serotype 19A
저자명
Jung. Seung-Jin,Seo. Eun-Seong,Yun. Sang-Il,Minh. Bui Nguyet,Jin. Sheng-De,Ryu. Hwa-Ja,Kim. Do-Man
간행물명
Journal of microbiology and biotechnology
권/호정보
2011년|21권 7호|pp.734-738 (5 pages)
발행정보
한국미생물생명공학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Streptococcus pneumoniae is a major cause of invasive infection in young infants and older adults. There are currently 90 capsular serotypes identified and 23 serotypes (1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19F, 19A, 20, 22F, 23F, and 33F) are responsible for about 90% of invasive disease. Among the more than 90 different S. pneumoniae serotypes, serotype 19A is globally very prevalent. A simplified purification procedure including adjustment of cell lysate pH to 4.5, fractionation with 50. 80% ethanol, and dialysis rendered capsular polysaccharide (CPS) in a yield of $31.32{pm}3.11$ mg from 1 l culture (75% recovery after lyses). The product contained only 69.6 ${mu}g$ of protein (99.78% purity) and 0.8mg (sum of the precipitants from 50~60%, 60~70%, and 70~80%) of nucleic acid (97.45% purity). The purified CPS was conjugated with bovine serum albumin; the product size ranged from 100 to 180 kDa.