This study was carried out to evaluate biological activities to different ethanol extracts from unripened and ripened fruit (Rubus coreanus Miq.). 25% to 75% ethanol extracts of unripened and ripened fruits were similar to extract yield respectively. Yield of ethanol extract of ripened fruit were approximately 3 times higher than that of unripened fruit. 75% ethanol extract of unripened fruit showed the highest contents of total polyphenol ($180.04{pm}0.41$ mg/g) and total flavonoid ($50.43{pm}0.81$ mg/g). Contents of total polyphenol and total flavonoid of unripened fruit were about 2 times higher than those of ripened fruit. $IC_{50}$ values of DPPH radical scavenging activity and linoleic acid peroxidation inhibition activity of BHA and ${alpha}$-tocopherol showed $13.19{pm}0.21$ and $18.16{pm}0.23{mu}g/m{ell}$, $4.25{pm}0.04{mu}g/m{ell}$ and $5.56{pm}0.10{mu}g/m{ell}$ respectively, but 75% ethanol extract of unripened fruit showed the lowest $23.85{pm}0.10{mu}g/m{ell}$ and $7.34{pm}0.07{mu}g/m{ell}$ among other all extracts. $IC_{50}$ values of LDL (low density lipoprotein) oxidation inhibition activity and ${alpha}$-glucosidase inhibitory activity of 75% ethanol extract of unripened fruit showed the lowest $1.04{pm}0.04{mu}g/m{ell}$ and $7.21{pm}0.13{mu}g/m{ell}$ among other all extracts respectively. Specifically, 75% ethanol extract of unripened fruit has relatively better biological activities than other ethanol extracts, it could be potentially used as bioactive source for health functional foods.