Vinegar has been considered as a potential organic herbicide, but more knowledge is needed about acetic acid concentration, application volume and use of adjutants on weed control. In this study, we determined optimum acetic acid concentration and spraying time on several weed species. Vinegar is more effective in broadleaf weeds than grasses. Vinegar is also effective in controlling younger weeds such as $3^{rd}$ to $4^{th}$ weeks after emergency. For example, $3^{rd}$ weeks after emergency weeds were easily controlled at 2-3% of vinegar solution. But $4^{th}$ weeks after emergency weeds were controlled at 4-5% of vinegar. Therefore vinegar concentration was recommended about 5%, and application time was when the weeds were young-within 3 leaf stage.