Measurement uncertainty analysis of fuel flow using turbine flowmeter was performed for the case of altitude engine test. SAE ARP4990 was used as the fuel flow calculation procedure, as well as the mathematical model for the measurement uncertainty assessment. The assessment was performed using Sensitivity Coefficient Method. 11 parameters involved in the calculation of the flow rate were considered. For the given equipment setup, the measurement uncertainty of fuel flow was assessed in the range of 1.19~1.86 % for high flow rate case, and 1.47~3.31 % for low flow rate case. Fluctuation in frequency signal from the flowmeter had the largest influence on the fuel flow measurement uncertainty for most cases. Fuel temperature measurement had the largest for the case of low temperature and low flow rate. Calibration of K-factor and the interpolation of the calibration data also had large influence, especially for the case of very low temperature. Reference temperature, at which the reference viscosity of the sample fuel was measured, had relatively small contribution, but it became larger when the operating fuel temperature was far from reference temperature. Measurement of reference density had small contribution on the flow rate uncertainty. Fuel pressure and atmospheric pressure measurement had virtually no contribution on the flow rate uncertainty.