This paper aims to build up a RFID-automated cargo movement system in an air-cargo warehouse. In our research, we do the spectrum measurements compliant with the RFID frequency bands to observe the interferences from legacy systems in the air-cargo warehouse. To overcome the dense-reader mode (DRM) problem, we apply three novel approaches, which are the periodic transmit of a reader, the Visible RFID reader & tag, and the sensor-based RFID system. Moreover, for the tag collision issue, we implement the Adaptive Adjustable Framed Q(AAFQ) algorithm, and the demonstration on RFID tag anti-collision algorithms show that the AAFQ algorithm has highly desirable tag anti-collision performance compared to any other commercially available solutions.