Following the appearance of the latest medical equipment with improved function, the importance of image analysis which enables effective image processing and analysis consistent with the hardware performance is on the rise. As well as, ongoing study is being done on the 2D medical image processing and 3D reconstruction. This paper segments chest CT images into each stage and finally shows 3D reconstruction of each segmented result. Among various image segmentation methods, Region Growing and apply sharpening and Gamma Controller as for image improvement for effective segmentation, image segmentation in order of bronchus and lung, bronchus, lung. Human organs image of segmented is use VTK(Visualization Toolkit) to make 3D reconstruction, two and three-dimensional medical image processing and analysis for lesions diagnosis are able to utilized.