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An Electrochemical Enzyme Immunochip Based on Capacitance Measurement for the Detection of IgG
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  • An Electrochemical Enzyme Immunochip Based on Capacitance Measurement for the Detection of IgG
  • An Electrochemical Enzyme Immunochip Based on Capacitance Measurement for the Detection of IgG
저자명
Yi. Seung-Jae,Choi. Ji-Hye,Kim. Hwa-Jung,Chang. Seung-Cheol,Park. Deog-Su,Kim. Kyung-Chun,Chang. Chulhun L.
간행물명
Bulletin of the Korean Chemical Society
권/호정보
2011년|32권 4호|pp.1298-1302 (5 pages)
발행정보
대한화학회
파일정보
정기간행물|ENG|
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기타
이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

This study describes the development of an electrochemical array immunochip for the detection of IgG. Interdigitated immunochip platforms were fabricated by sputtering gold on a glass wafer by using MEMS process and then were coated with Eudragit S100, an enteric polymer, forming an insulating layer over the working area of immunochips. The breakdown of the polymer layer was exemplified by the catalytic action of urease which, in the presence of urea, caused an alkaline pH change. This subsequently caused an increase of the double layer capacitance of the underlying electrode. Used in conjunction with a competitive immunoassay format, this allowed the ratio of initial to final electrode capacitance to be directly linked with the concentration of analyte, i.e. IgG. Responses to IgG could be detected at IgG concentration as low as $250;ngmL^{-1}$ and showed good linearity up to IgG concentration as high as $20;{mu}gmL^{-1}$.