In this paper, we propose a 3D conversion algorithm of 2D video using depth layer partition method. In the proposed algorithm, we first set frame groups using cut detection algorithm. Each divided frame groups will reduce the possibility of error propagation in the process of motion estimation. Depth image generation is the core technique in 2D/3D conversion algorithm. Therefore, we use two depth map generation algorithms. In the first, segmentation and motion information are used, and in the other, edge directional histogram is used. After applying depth layer partition algorithm which separates objects(foreground) and the background from the original image, the extracted two depth maps are properly merged. Through experiments, we verify that the proposed algorithm generates reliable depth map and good conversion results.