Purpose: This study investigated the effect of needle electrode stimulation at various frequencies on change in wound healing in excision wound rats Methods: Twenty-four Sprague-Dawley adult male rats were assigned to one of four groups: control (n=6), acupuncture group (n=6), low rate (2 Hz) percutaneous electrical stimulation group, high rate (100 Hz) percutaneous electrical stimulation group (n=6). We analyzed morphological effects by measuring the area of the excision wound, the contraction rate, and chromatic red. A digital camera and an image analysis program were used to measure and analyze the wound area,which was also used for the contraction rate. Chromatic red was obtained by calculating red, green, and blue (RGB) values of the wound area. Results: The electro acupuncture stimulation groupsshowed significant healing effects compared to the control and acupuncture groups. Conclusion: The results of this study showed that various frequencies of percutaneous electrical stimulation have a therapeutic effect on wound healing.