Purpose: This study aimed to analyze factors that influence the job satisfaction of physical therapists working in hospitals. Methods: The study was done using a self-administered questionnaire associated with job satisfaction, which was completed by 352 physical therapists working in hospitals. Results: The average job satisfaction score was 3.16. Sub-items scores for job satisfaction were higher for overall satisfaction (3.21) and lower for job satisfaction of achievement (3.12) than the average score. Job satisfaction of subjects showed statistically significant differences with regard to differences in sex, marriage, type of hospital, year of total career, type of patients and motivation level (p<0.05). The average job stress score was 2.38. The sub-items of job stress consisted of problems pertaining to physical environment (2.28), job demand (2.54), job autonomy (2.31), social support (2.14), job instability (2.36), organized management system (2.44), inner motive (2.24), and job culture (2.26). Multiple regression analysis revealed that job satisfaction became significantly lower with increasing levels of job stress. Conclusion: These results suggest that the job stress of physical therapists is closely related to their job satisfaction. An effort to reduce job stress may be helpful in improving the health and quality of life of physical therapists who work in hospitals.