The phenol amount of Lygodium japonicum extracted by the pressure-assisted water extraction (PAWE) was noticeably increased to 12.8mg GAE/g when compared to the conventional extraction (9.3 mg GAE/g) (p<0.05). The highest antioxidant activity was observed at Polygonum aviculare extracted by the PAWE, showing the lowest EC50 value of 0.09 mg/mL. The lowest minimum inhibitory concentrations ($MIC_{99}$) of L. japonicum extracted by the PAWE were 2.59, 3.33, and 7.37 mg/mL, respectively, against Listeria monocytogenes, Salmonella Typhimurium, and Propionibacterium acnes. These results suggest that the PAWE can be an appropriate extraction method for enhancing biological and pharmaceutical properties of medicinal plants.