Objectives: The purpose of this study was to investigate the effects of Angelicae pubescentis Radix water extract (ACE) on immune properties in macrophage cells. Methods: The cells were divided into two groups: As a control, the first was not treated with ACE, and the other was treated with ACE. Together with the cell viability, productions of nitric oxide (NO) and cytokines such as interleukin (IL)-$1{eta}$, IL-6, and tumor necrosis factor (TNF)-${alpha}$ by treating of ACE were monitored. Results: 1. There was no decrease of the cell viability after 24 hr incubation, but a significant decrease after 48 hr incubation with all four concentrations (25, 100, 200, and $400;{mu}g/m{ell}$) of ACE. 2. A significant increase in the production of NO was observed in the concentrations above $50;{mu}g/m{ell}$ of ACE after 24 hr incubation. 3. Further, after 48 hr incubation, the critical concentration of ACE for the increase was reduced to $25;{mu}g/m{ell}$. 4. The production of (IL)-$1{eta}$ significantly increased with the ACE concentrations of 100 and $200;{mu}g/m{ell}$ after 24 hr incubation. 5. The production of IL-6 significantly increased with the ACE concentration of $200;{mu}g/m{ell}$ after 24 hr incubation. 6. A significant increase in the production of (TNF)-${alpha}$ was detected with ACE concentrations of 50, 100, and $200;{mu}g/m{ell}$ after 24 hr incubation. Conclusions: These show that ACE increases mouse macrophage NO production at concentrations above $50;{mu}g/m{ell}$, and the cytokines ((IL)-$1{eta}$, IL-6, and (TNF)-${alpha}$) at concentrations above $200;{mu}g/m{ell}$. These results suggest that ACE improves macrophage immune property.