Background and Objectives : Clinically, as a tool for voice assessment before and after the operation or the voice treatment, acoustic analysis is widely used. However, in clinical situations, acoustic parameters vary according to how the assessment is made. Thus, with voice disease patients as subjects, we are to investigate what influence intensity increase exerts on acoustic parameters and how to reduce variation according to the way of assessing. Material and Method : At the voice clinic of the department of otorhinolaryngology in Gangnam Severance Hospital, with 30 female voice-disease patients (40.6 years old on the average) and 23 male voice-disease patients (40.1 years old on the average) as subjects, using the Dr Speech vocal-assessment program, we statistically tested the significance of the difference in each of acoustic parameters between when the "Ah" vowel is produced with a normal voice and when the "Ah" vowel is produced with a loud voice. Results : Acoustic parameters that showed a statistically significant difference according to intensity increase were Jitter, SD F0, and NNE for females, and Jitter, SD F0, HNR, SNR, and NNE for males. Voice quality estimates showed a statistically significant difference according to intensity increase in female hoarse voice, female breathy voice, and male breathy voice. Conclusion : In this research, acoustic analysis, which is generally used for voice assessment before and after the operation or the voice treatment, showed a tendency that acoustic parameters became better under the influence of intensity increase except for the cases where a voice disease was severe. Thus, to raise the reliability of voice assessment, the range of intensity needs to be set up. This should be the topic for the future research.