In order to get dynamic serviceability of a train travelling on a railway bridge, comfort limits with the deflection of bridge and vertical acceleration on car body are proposed in Eurocode, Shinkansen design criteria, The design guideline of the Honam High-speed railway. The design guideline of the Honam High-speed railway has quoted Eurocode. Therefore it is expected that supplementation of comfort limit of railway bridge according to expansion of span length and the improvement traveling speed of trains in the future would relatively fall behind developed countries in railway. Therefore, in order to secure technological competitiveness in world market, the study was conducted to propose the deflection limit based on vibration serviceability of railway bridges that can consider bridge-train interaction and travelling speed increase. The parameter study and bridge-train dynamic interaction analysis was conducted to figure out the correlation of vertical acceleration on car body and bridge displacement according to the increase in travelling speed. Also, the trend of increasing vertical acceleration on car body according to the increase in travelling speed was confirmed, and the amplification coefficient of vertical acceleration on car body was suggested. And the deflection form and vibration of the bridge were assumed to be in harmonic motion, and transfer function and the amplification coefficient were used to develop the dynamic serviceability deflection limit of the high-speed railway bridge as a formula.