A model test is carried out to investigate the vibration of truss type lift gate in the four major rivers project. The gate model scaled with the ratio of 1 : 25 is made of acryl panel dimensioned 1.6 m in width, 0.28 m in height in the concrete test flume. Firstly natural frequencies of the model gate are measured and the results are compared with the numerical results in order to verify the model. The amplitudes of the vibration are measured under the different gate opening and water level conditions. The results are analyzed to study the characteristics of the gate vibration according to the small gate opening, the large gate opening and the overflow conditions. These test results presents a basic data for the guide manuals of gate management and a design method to reduce the gate vibration of truss type lift gate. Finally, the vibration of truss type lift gate are assessed in comparison with those of formerly tainter gate.