A new diterpene glycoside, tomentoside I (1), along with eleven known compounds, including the four coumarins, 4,5-dimethoxyl-7-methylcoumarin (2), 4,7-dimethoxyl-5-methylcoumarin (3), isofraxidin (4) and fraxidin (5) as well as the seven triterpenoids, oleanolic acid (6), oleanolic acid 3-O-${alpha}$-L-arabinopyranoside (7), oleanolic acid 3-O-${eta}$-D-galactopyranosyl-($1{ ightarrow}3$)-${eta}$-Dglucopyranoside (8), hederagenin 3-O-${alpha}$-L-arabinopyranoside (9), betulinic acid (10), 18-hydroxyursolic acid (11) and $2{alpha}$,$3{eta}$,23-trihydroxyurs-12-en-28-oic acid (12) were isolated from the ethanolic extract of the root of Anemone tomentosa and their chemical structures were elucidated by spectroscopic methods. The antimicrobial activities of compounds 1-12 were measured using the agar disc-diffusion method. Also, their antioxidant activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) were evaluated.