The Spectral Fatigue Analysis is representative fatigue life assessment method for vessels. This Analysis is performed generally for the whole vessel and many assessment sites. The spectral fatigue analysis is performed through the process of hydrodynamic response analysis, global structural analysis, local structural analysis and calculation of fatigue damage. In these processes, fatigue damage is affected by many variables. The representative variables are S-N curve data, wave scatter data, wave spectrum, bandwidth effect and etc. In this paper, the effects of these variables to the fatigue damage are analyzed through the spectral fatigue analysis for 170k LNGC.