Background Clinafloxacin dithiocarbamate (CNND) was radiolabeled with technetium-99m ($^{99m}Tc$) using $[^{99m}Tc(CO){_3}(H_2O){_3}]^+$ and assessed for its radiochemical stability in saline and serum, its in vitro binding with methicillinresistant Staphylococcus aureus (MRSA) and biodistribution in female nude mice (FNM) artificially infected with live and heat-killed MRSA. Methods In normal saline (NS) the $^{99m}Tc(CO){_3}$-clinafloxacin dithiocarbamate ($^{99m}Tc(CO){_3}$-CNND) showed radiochemical stability with a maximum value of $99.10{pm}0.20%$ and remained stable up to 4 h ($92.65{pm}0.18%$). Results In human serum at $37^{circ}C$ within 16 h of incubation, 14.85% side products as a result of de-tagging developed. Incubation with MRSA gave saturated binding with a maximum value of $72.75{pm}1.20%$. Almost six-fold higher uptake was seen in the infected muscle of the FNM as compared to the inflamed and normal muscle. The $^{99m}Tc(CO){_3}$-CNND complex showed a normal route of excretion from the body of the FNM model. Conclusion The higher stability in NS, HS, saturated in vitro binding with a live strain ofMRSA and six-fold higher uptake in the target organ showed the $^{99m}Tc(CO){_3}$-CNND complex to be a potential MRSA infection radiotracer.