A Gram-positive, rod-shaped, motile, endospore-forming bacterial strain, designated $NB22^T$, was isolated from soil of a lettuce field in Kyonggi province, South Korea, and was characterized by using a polyphasic taxonomic approach. This novel isolate grew optimally at $30-37^{circ}C$ and pH 8-9. It grew in the presence of 0-4% NaCl (optimum, 1-2%). Comparative 16S rRNA gene sequence analysis showed that strain $NB22^T$ was closely related to members of the genus Bacillus and fell within a coherent cluster comprising B. siralis $171544^T$ (98.1%) and B. korlensis ZLC-$26^T$ (97.3%). The levels of 16S rRNA gene sequence similarity with respect to other Bacillus species with validly published names were less than 96.4%. Strain $NB22^T$ had a genomic DNA G+C content of 36.3 mol% and the predominant respiratory quinone was MK-7. The peptidoglycan contained meso-diaminopimelic acid. The major cellular fatty acids were iso-$C_{15:0}$, anteiso-$C_{15:0}$, $C_{14:0}$, and $C_{16:0}$. These chemotaxonomic results supported the affiliation of strain $NB22^T$ to the genus Bacillus, and the low DNA-DNA relatedness values and distinguishing phenotypic characteristics allowed genotypic and phenotypic differentiation of strain $NB22^T$ from recognized Bacillus species. On the basis of the evidence presented, strain $NB22^T$ is considered to represent a novel species of the genus Bacillus, for which the name Bacillus kyonggiensis sp. nov. is proposed. The type strain is $NB22^T$ (=KEMB 5401-$267^T$ =JCM $17569^T$).