Enzymes are being extensively used in industry as they are environmentally friendly. They show a variety of advantages over chemicals, like their specificity, high efficiency and eco-friendliness. Nowadays different kinds of cellulase enzyme mixtures are commercially formulated for the finishing of cellulosic fabrics. This study investigated the effect of acid cellulase treatment on bamboo knitted-fabric to reduce pill. It has been observed that specific range of concentration only can effectively reduce pills with substantial weight loss. The common process and method followed by a textile chemist to optimize or standardize the concentration of enzyme treatment to reduce pill is still cumbersome and doubtful. A quicker and objective method is proposed to optimize the concentration of enzyme. The effectiveness is evaluated using standard Martindale tester and the weight loss of the fabric.