This study was performed to investigate the bioactivity of sancho (Zanthoxylum schinifolium) essential oil (EO) on bone metabolism and its function of osteoblastic MC3T3-E1 cells. The volatile aroma components of sancho EO were collected using a Clevenger-type apparatus by steam distillation extraction method, and determined by GC-MS. ${eta}$-Phellandrene (22.54%) was the most abundant volatile compound in sancho EO, followed by citronellal (16.48%) and geranyl acetate (11.39%). It increased the collagen and mineralization of osteoblasts (p<0.05), indicating that sancho may help prevent osteoporosis.