작약 연작장해 경감대책을 마련하기 위하여 연작재배지의 토양 이화학성과 미생물상 변화 및 토양 미생물 군락의 기질 이용성을 조사하였다. 작약 초작지 토양에 비해 연작지 토양에서 표토 깊이가 얕고 표토와 심토의 경도차이가 심하였다. 토양화학성에서는 초작 2년에 비해 연작 4년생 토양이 pH 7.6, 유기물함량 $44.1g;kg^{-1}$, 치환성 Ca, Mg함량이 높았다. 작약 재배연수에 따른 미생물의 변화는 세균과 방선균 밀도는 초작 3년까지 증가하다가 그 이후 감소하였고 사상균 밀도는 재배년수에 따라 크게 증가하였다. Biolog EcoPlate에 의한 토양 미생물 군락의 탄소기질 이용성 차이는 제 3주성분까지 57.5%의 자료를 해석할 수 있었고, 주성분에 영향을 미치는 탄소기질은 D-cellobiose, ${eta}$-methyl-D-glucoside 등이었으며, 작약 재배기간이 길어짐에 따라 뚜렷하게 구분되었다.
This study was conducted to obtain the information about injury caused by continuous cropping of peony (Paeonia lactiflora). Soil physico-chemical properties, characteristics of microbial distribution and diversities in the continuous cropped field with peony were analyzed. As the results, pH and organic matter content were higher in the continuous cropping soil than those in the first cropping soil. Bulk density was decreased but porosity was increased in the continuous cropping soil. As the cultivation period was lengthened in years, the populations of bacteria and actinomyces were gradually decreased, whereas fungal population was increased. It was shown that the metabolic diversity patterns of the microbial communities in the continuous cropping soil differed from that of the first cropping soil. These results indicate that deterioration of soil quality such as physico-chemical properties including a soil depth, bulk density, porosity and soil pH is related with a continuous cultivation periods, and also affect a microbial population, especially fungi.
