In this study, a 3-D CFD simulation and laser diagnostics were developed to understand the characteristics of soot generation in a diesel diffusion flame. The recently developed RANS (Reynolds-averaged Navier-Stokes equations) hybrid combustion model (Extended Coherent Flame Model - 3 Zones, ECFM-3Z model) was used. This industrial, state-of-the-art model of the diffusion flame is commonly used in diesel combustion models as well as for propagating (premixed) flame combustion. The simulation results were validated with measurements from a constant volume combustion chamber. The experiment revealed that soot accumulated in the chamber where the temperature decreased. Where the temperature increased rapidly, only a little soot accumulated. The temperature and soot distribution were independently examined using both the two-color method and a 3-D CFD simulation for a turbulent diesel diffusion flame.