A series of 1-(2,6-dichloro-4-trifluoromethylphenyl)-1H-1,2,3-triazole analogues have been tested for antimicrobial activity against thirteen bacteria and three fungi in order to observe the broad spectrum activity. Among the compounds tested, the C-4-chloropropyl derivative exhibited potent antibacterial and antifungal activities. The C-4-substituted regioisomers of 1-(2,6-dichloro-4-trifluoromethylphenyl)-1H-1,2,3-triazole exhibited moderate selectivity to broad spectrum activity, compared to that of 5-substituted regioisomers.