Objectives : The purpose of this study is to compare the effect of superficial acupuncture to deep acupuncture on normal muscle function. Methods : In Group A, 12 healthy subjects had been treated by deep acupuncture(10mm). In Group B, 12-healthful subjects had been treated by superficial acupuncture(5mm). Each session took 10 minutes. Before and after the treatment, outcomes were assessed by Algometer for muscle tenderness, dynamometer for grasping power and Meridian-Electromyograph(MEMG) for extensor muscles. Results : The Contraction power of finger extensor muscles assessed by MEMG had decreased significantly in both groups. The muscle fatigue of finger extensor muscles assessed by MEMG had increased significantly in Deep acupuncture group. Grasping power assessed by dynamometer had no significant change in both groups. Conclusions : According to above results, superficial acupuncture is more effective on reducing muscle fatigue.