A fuel injection system which is operated with a real vehicle driving simulation was developed as an alternative to a vehicle test for the fuel injectors. The sensor signals that are supplied to the ECU were measured and recorded as a data file for a vehicle driven in FTP-75 mode in a chassis dynamometer. The imperative sensor signals of the throttle position, vehicle speed, engine speed, crank position, cam position, intake air flow, and cooling water and intake air temperature were reconstructed using FPGA DAQ boards and a PXI computer. The scanning results showed good agreement with the input signals that were reconstructed. The ECU HILS system operated successfully to drive six fuel injectors, which injected fuel in the same pattern as if they were mounted in the vehicle driven in FTP-75 mode. Also, the fuel injection system developed in this research shows the possibility of application in evaluating the characteristics of fuel injection rate for injectors according to properties of injected fuel with the real driving mode of vehicles.