In this paper, we have introduced a Missile In the Loop Simulation(MILS) Software developed for the missile ground test, which is based on a commercial hard real-time operating system(OS) on Windows platform called as Real-Time eXtension(RTX). MILS software makes it possible to test overall system functions of a integrated missile on the ground in the flight conditions by real-time imitating its inertial data. By means of MILS, we have performed missiles ground tests, which result in successful real flight tests.