Recently arithmetic circuit of lightweight AES symmetric key algorithm that can apply to passive tag have been developed, then security protocol of RFID system using AES symmetric encryption techniques have been proposed. This paper proposed security enhancement protocol of RFID system using lightweight AES arithmetic circuit and random number generator of passive tag. The proposed protocol have AES algorithm and random number generator at server, reader, tag, and transmit encrypted message by separate secret key using random number at each session. The mutual authentication of tag and reader used reader random number and tag random number. As a result, proposal protocol reduce authentication steps of the existing mutual authentication protocol, and reduce amount of computation of tag, and demonstrate as secure protocol to every attack type of attacker by decrease communication step of Air Zone.