This paper describes the autonomous operation analysis of DC microgrid based on droop control. In order to verify the whole system operation, detailed simulation models for wind power generation, solar power generation, and battery were developed with user-defined models programmed with C-code in PSCAD/ EMTDC software. The simulation results confirm that the DC microgrid with droop control make it feasible to provide power to the load with stable manner. Based on simulation results a prototype of DC microgrid was built and tested in the lab to verify the autonomous operation experimentally. The droop control scheme can suppress the circulating current, and offers each unit to be controlled autonomously without any communication link.