기관회원 [로그인]
소속기관에서 받은 아이디, 비밀번호를 입력해 주세요.
개인회원 [로그인]

비회원 구매시 입력하신 핸드폰번호를 입력해 주세요.
본인 인증 후 구매내역을 확인하실 수 있습니다.

회원가입
서지반출
Amenability of Castor to an Agrobacterium- Mediated in planta Transformation Strategy Using a cry1AcF Gene for Insect Tolerance
[STEP1]서지반출 형식 선택
파일형식
@
서지도구
SNS
기타
[STEP2]서지반출 정보 선택
  • 제목
  • URL
돌아가기
확인
취소
  • Amenability of Castor to an Agrobacterium- Mediated in planta Transformation Strategy Using a cry1AcF Gene for Insect Tolerance
  • Amenability of Castor to an Agrobacterium- Mediated in planta Transformation Strategy Using a cry1AcF Gene for Insect Tolerance
저자명
Kumar. Arthikala Manoj,Sreevathsa. Rohini,Reddy. Kalpana Nanja,Ganesh. Prasa Trichy,Udayakumar. Makarla
간행물명
Journal of crop science and biotechnology
권/호정보
2011년|14권 2호|pp.125-132 (8 pages)
발행정보
한국작물학회
파일정보
정기간행물|ENG|
PDF텍스트
주제분야
기타
이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

Agrobacterium tumefaciens mediated in planta transformation protocol was developed for castor, Ricinus communis. Two-day-old seedlings were infected with Agrobacterium strain EHA105/pBinBt8 harboring cry1AcF and established in the greenhouse. Screening the $T_1$ generation seedlings on 300 mg $L^{-1}$ kanamycin identified the putative transformants. Molecular and expression analysis confirmed the transgenic nature and identified high-expressing plants. Western blot analysis confirmed the co-integration of the nptII gene in the selected transgenic plants. Bioassay against Spodoptera litura corroborated with high expression and identified five promising effective lines. Analysis of the $T_2$ generation plants proved the stability of the transgene indicating the feasibility of the method.