In this paper, we propose a novel focus measure that determines in-focus and out-of-focus region in an image. In addition, we achieved extended depth of field by blending the acquired image and Wiener filtered image using a decision map based on the designed focus measure. Since conventional focus measures are based on the amount of high frequency components in an acquired image, the measures may not be accurate if there exist high frequency components in out-of-focused region. To overcome the problem, we designed the novel focus measure based on effective band pass filtering. In simulations and experiments, the proposed method showed better performance than existing methods.