Purpose of this study was to evaluate germination characteristics of soil seed bank in rural stream topsoil using seedling emergence method in order to provide data for future ecological restoration of stream utilizing topsoil. There were 24 families, 52 genera, 61 taxa of soil seed bank flora found in topsoil from 6 rural streams. The most frequently found taxa were Compositae (12 taxa) followed by Gramineae (8 taxa), Caryophyllaceae (5 taxa), Cruciferae (4 taxa), Scrophulariaceae, Labiatae, Polygonaceae and Cyperaceae. Plant with the most number of germination was Stellaria aquatica followed by Erigeron annuus, Imperata cylindrica var. koenigii, Poa annua, Cyperus microiria and Veronica undulata. Naturalized plants found were Erigeron annuus, Rumex crispus, Oenothera odorata, Cerastium glomeratum, Bidens frondosa, Erigeron philadelphicus, etc.